IDENTIFICATION OF AN IMMUNODOMINANT EPITOPE WITHIN THE CAPSID PROTEIN OF HEPATITIS-C VIRUS

被引：116

作者：

NASOFF, MS

ZEBEDEE, SL

INCHAUSPE, G

PRINCE, AM

机构：

[1] PHARMACIA GENET ENGN INC,LA JOLLA,CA 92037

[2] NEW YORK BLOOD CTR,VIROL & PARASITOL LAB,NEW YORK,NY 10021

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 12期

关键词：

GLUTATHIONE S-TRANSFERASE; FUSION PROTEINS; EPITOPE MAPPING; IMMUNOBLOT; ELISA;

D O I：

10.1073/pnas.88.12.5462

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have isolated cDNA clones from the 5' end of the Hutchinson strain of hepatitis C virus. Sequences encoding various segments of the HCV structural region were fused to the gene for glutathione S-transferase and analyzed for the expression of hepatitis C virus-capsid fusion proteins. With a set of these fusion proteins, both human and chimpanzee immune responses to capsid were studied. An immunodominant epitope was located within the amino-terminal portion of capsid that is preferentially recognized by antibodies in both human and chimpanzee hepatitis C virus-positive sera. In addition, analyses of sequential serum samples taken from humans and chimpanzees with either chronic or apparently self-limited infections revealed that a strong anti-capsid response develops rapidly after onset of infection.

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页码：5462 / 5466

页数：5

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