DETERMINATION OF I-125 LABELED THYROXINE GLUCURONIDE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ONLINE RADIOCHEMICAL DETECTION TO DETERMINE UDPGLUCURONOSYLTRANSFERASE ACTIVITY

被引：11

作者：

DUCROTOY, G ^{[1
]}

RICHERT, L ^{[1
]}

DESANDRO, V ^{[1
]}

LURIER, D ^{[1
]}

PACAUD, E ^{[1
]}

机构：

[1] CTR RECH VITRY ALFORTVILLE,RHONE POULENC RORER,INST RECH SECUR MED,F-94403 VITRY,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 566卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80258-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A convenient, fast and highly sensitive high-performance liquid chromatographic method, using online radiochemical detection, is described for the determination of [I-125]thyroxine glucuronide. The method involves direct injection of the supernatant, a total analysis time of 30 min and a detection limit of 1 pmol. The results demonstrate that the method is suitable for the determination of UDPglucuronosyltransferase activity with thyroxine as substrate in native hepatic microsomes. The rate of thyroxine glucuronidation in microsomes from rats treated with Arodor 1254 was ten times higher than in control microsomes, indicating that with this method, increases of UDPglucuronosyltransferase thyroxine activities, often associated with hepatic induction process involved in thyroid hypertrophy, can be easily detected. This method could also be applied to all experimental biological systems that involve the separation and quantification of [I-125]thyroxine and [I-125]thyroxine glucuronide.

引用

页码：415 / 426

页数：12

共 20 条

[1] POLYCHLORINATED BIPHENYLS - NEW TYPE OF INDUCER OF CYTOCHROME P-448 IN LIVER - (CYTOCHROME P-450 RATS ARYL HYDROCARBON HYDROXYLASE-ENZYME INDUCTION MICROSOMAL ENZYMES) [J].

ALVARES, AP ;

BICKERS, DR ;

KAPPAS, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (05) :1321-1325

[2]

AMRI HS, 1988, TOXICOL APPL PHARM, V92, P141

[3] ENHANCED INVITRO HEPATIC GLUCURONIDATION OF THYROXINE IN RATS FOLLOWING CUTANEOUS APPLICATION OR INGESTION OF POLYCHLORINATED BIPHENYLS [J].

BASTOMSKY, CH ;

MURTHY, PVN .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1976, 54 (01) :23-26

[4] EFFECTS OF A POLYCHLORINATED BIPHENYL MIXTURE (AROCLOR-1254) AND DDT ON BILIARY THYROXINE EXCRETION IN RATS [J].

BASTOMSKY, CH .

ENDOCRINOLOGY, 1974, 95 (04) :1150-1155

[5] UDP GLUCURONYLTRANSFERASE ACTIVITY IN ISOLATED PERFUSED RAT-LIVER [J].

BOCK, KW ;

FROHLING, W .

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 1973, 277 (01) :103-106

[6]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[7] CHANGES IN THYROIDAL FUNCTION AND LIVER UDPGLUCURONOSYLTRANSFERASE ACTIVITY IN RATS FOLLOWING ADMINISTRATION OF A NOVEL IMIDAZOLE (SC-37211) [J].

COMER, CP ;

CHENGELIS, CP ;

LEVIN, S ;

KOTSONIS, FN .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1985, 80 (03) :427-436

[8] KINETIC-STUDIES OF LATENT MICROSOMAL UDP-GLUCURONYLTRANSFERASES - KINETICS OF GLUCURONIDATION IN INTACT AND PERTURBANT-TREATED MEMBRANES [J].

CUMMINGS, J ;

GRAHAM, AB ;

WOOD, GC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 771 (02) :127-141

[9] PREPARATION AND SEPARATION OF THE GLUCURONIDE AND SULFATE CONJUGATES OF THYROXINE AND TRIIODOTHYRONINE [J].

HAYS, MT ;

HSU, L .

ENDOCRINE RESEARCH, 1987, 13 (03) :215-228

[10] HIGH-PRESSURE LIQUID-CHROMATOGRAPHY OF AMINO-ACIDS, PEPTIDES AND PROTEINS .5. SEPARATION OF THYROIDAL IODO-AMINO ACIDS BY HYDROPHILIC ION-PAIRED REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

HEARN, MTW ;

HANCOCK, WS ;

BISHOP, CA .

JOURNAL OF CHROMATOGRAPHY, 1978, 157 (SEP) :337-344

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