A FIBER-BED BIOREACTOR FOR ANCHORAGE-DEPENDENT ANIMAL-CELL CULTURES .1. BIOREACTOR DESIGN AND OPERATIONS

被引：46

作者：

CHIOU, TW

MURAKAMI, S

WANG, DIC

WU, WT

机构：

[1] MIT,CTR BIOTECHNOL PROC ENGN,CAMBRIDGE,MA 02139

[2] NATL TSING HUA UNIV,HSINCHU 300,TAIWAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 08期

关键词：

CELL CULTURE; FIBER-BED BIOREACTOR; ANCHORAGE-DEPENDENT CELL CULTURES; AIRLIFT; BIOREACTOR DESIGN;

D O I：

10.1002/bit.260370810

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A concentric-cylinder airlift reactor, in which the annulus is a packed bed of glass fibers, has been developed in order to facilitate the scaleup and enhance the volumetric productivity of anchorage-dependent animal cell cultures. In this bioreactor, oxygen-containing gas is sparged through the inner draft tube, causing bubble-free medium to flow through the fiber bed in the outer cylinder and providing both oxygenation and convective nutrient transfer to the cells. Several other desirable features for reactor operation are also provided by this design. Cell cultivations in this bioreactor have been successfully carried out and provide data for the feasibility of the large-scale cell cultivation.

引用

页码：755 / 761

页数：7

共 12 条

[1] THE ADHESION OF ANIMAL-CELLS TO SURFACES - THE MEASUREMENT OF CRITICAL SURFACE SHEAR-STRESS PERMITTING ATTACHMENT OR CAUSING DETACHMENT [J].