TOPOLOGY OF EUKARYOTIC TYPE-II MEMBRANE-PROTEINS - IMPORTANCE OF N-TERMINAL POSITIVELY CHARGED RESIDUES FLANKING THE HYDROPHOBIC DOMAIN

被引:150
作者
PARKS, GD [1 ]
LAMB, RA [1 ]
机构
[1] NORTHWESTERN UNIV,HOWARD HUGHES MED INST,EVANSTON,IL 60208
关键词
D O I
10.1016/0092-8674(91)90507-U
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have tested the role of different charged residues flanking the sides of the signal/anchor (S/A) domain of a eukaryotic type II (N(cyt)C(exo)) integral membrane protein in determining its topology. The removal of positively charged residues on the N-terminal side of the S/A yields proteins with an inverted topology, while the addition of positively charged residues to only the C-terminal side has very little effect on orientation. Expression of chimeric proteins composed of domains from a type II protein (HN) and the oppositely oriented membrane protein M2 indicates that the HN N-terminal domain is sufficient to confer a type II topology and that the M2 N-terminal ectodomain can direct a type II topology when modified by adding positively charged residues. These data suggest that eukaryotic membrane protein topology is governed by the presence or absence of an N-terminal signal for retention in the cytoplasm that is composed in part of positive charges.
引用
收藏
页码:777 / 787
页数:11
相关论文
共 49 条
[1]   INCORPORATION OF A CHARGED AMINO-ACID INTO THE MEMBRANE-SPANNING DOMAIN BLOCKS CELL-SURFACE TRANSPORT BUT NOT MEMBRANE ANCHORING OF A VIRAL GLYCOPROTEIN [J].
ADAMS, GA ;
ROSE, JK .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (06) :1442-1448
[2]  
AKITA M, 1990, J BIOL CHEM, V265, P8164
[3]   INTRACELLULAR PROTEIN TOPOGENESIS [J].
BLOBEL, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1496-1500
[4]   TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].
BLOBEL, G ;
DOBBERSTEIN, B .
JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851
[5]   ACCESS OF PROTEINASE-K TO PARTIALLY TRANSLOCATED NASCENT POLYPEPTIDES IN INTACT AND DETERGENT-SOLUBILIZED MEMBRANES [J].
CONNOLLY, T ;
COLLINS, P ;
GILMORE, R .
JOURNAL OF CELL BIOLOGY, 1989, 108 (02) :299-307
[6]   THE SPONTANEOUS INSERTION OF PROTEINS INTO AND ACROSS MEMBRANES - THE HELICAL HAIRPIN HYPOTHESIS [J].
ENGELMAN, DM ;
STEITZ, TA .
CELL, 1981, 23 (02) :411-422
[7]  
ERICKSON AH, 1979, J BIOL CHEM, V254, P1771
[8]   PURIFICATION OF MICROSOMAL SIGNAL PEPTIDASE AS A COMPLEX [J].
EVANS, EA ;
GILMORE, R ;
BLOBEL, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (03) :581-585
[9]   EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].
FUERST, TR ;
NILES, EG ;
STUDIER, FW ;
MOSS, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126
[10]   TRANSLOCATION OF SECRETORY PROTEINS ACROSS THE MICROSOMAL MEMBRANE OCCURS THROUGH AN ENVIRONMENT ACCESSIBLE TO AQUEOUS PERTURBANTS [J].
GILMORE, R ;
BLOBEL, G .
CELL, 1985, 42 (02) :497-505