ANTIBACTERIAL ACTIVITY OF EISENIA-FOETIDA-ANDREI CELOMIC FLUID - IMMUNOLOGICAL STUDY OF THE 2 MAJOR ANTIBACTERIAL PROTEINS

被引：13

作者：

HIRIGOYENBERRY, F

LASSEGUES, M

ROCH, P

机构：

[1] Département de Physiologie des Invertébrés, Université de Bordeaux I, URA CNRS 04, 33405 Talence Cedex, 1138, Avenue des Facultés

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1992年 / 59卷 / 01期

关键词：

ANNELID; HUMORAL IMMUNITY; ANTIBACTERIAL PROTEINS; IMMUNOLOGICAL STUDY;

D O I：

10.1016/0022-2011(92)90113-I

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Antibacterial activity of Eisenia fetida andrei coelomic fluid was mainly mediated by two proteins named 45 kDa and 40 kDa. These two molecules were purified under denaturing conditions (SDS, 2-mercaptoethanol) and two polyclonal antibodies were raised. Antibodies were characterized by ELISA, Western blotting, and dot blotting. They raised the same titer ( 1 40,000), and their limits of detection were almost identical. The specificity of each antibody was also determined and a cross-reactivity between 45- and 40-kDa defense proteins has been observed. Partial amino acid sequence homologies is discussed. © 1992.

引用

页码：69 / 74

页数：6

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