SEPARATION AND IDENTIFICATION OF CYTOKININS USING COMBINED CAPILLARY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：19

作者：

IMBAULT, N ^{[1
]}

MORITZ, T ^{[1
]}

NILSSON, O ^{[1
]}

CHEN, HJ ^{[1
]}

BOLLMARK, M ^{[1
]}

SANDBERG, G ^{[1
]}

机构：

[1] SWEDISH UNIV AGR SCI,DEPT FOREST GENET & PLANT PHYSIOL,S-90183 UMEA,SWEDEN

来源：

BIOLOGICAL MASS SPECTROMETRY | 1993年 / 22卷 / 03期

关键词：

D O I：

10.1002/bms.1200220310

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Non-derivatized cytokinins were analysed by liquid chromatography/mass spectrometry (LC/MS). The effluent from a capillary reversed-phase high-performance liquid chromatography column was introduced into the ion source of a double-focusing mass spectrometer via a frit interface, and ions were generated by fast atom bombardment (FAB) with 1% glycerol in the mobile phase acting as a matrix. Positive FAB spectra were obtained for base, riboside, ribotide and glucoside forms of cytokinins. The spectra were found to provide useful information for identification and structural elucidation of cytokinins. The LC/MS system was used to identify iso-pentenyladenosine in a purified extract from Norway spruce (Picea abies) needles. Quantitative analysis of iso-pentenyladenosine using (H-2(6))isopentenyladenosine as internal standard indicated levels of 1.2 ng g-1 fresh weight.

引用

页码：201 / 210

页数：10

共 16 条

[1] ETHYLENE ACCELERATES THE BREAKDOWN OF CYTOKININS AND THEREBY STIMULATES ROOTING IN NORWAY SPRUCE HYPOCOTYL CUTTINGS [J].

BOLLMARK, M ;

ELIASSON, L .

PHYSIOLOGIA PLANTARUM, 1990, 80 (04) :534-540

[2] VARIATION IN ENDOGENOUS CYTOKININ CONTENT DURING ADVENTITIOUS ROOT-FORMATION IN PEA CUTTINGS [J].

BOLLMARK, M ;

KUBAT, B ;

ELIASSON, L .

JOURNAL OF PLANT PHYSIOLOGY, 1988, 132 (03) :262-265

[3] FAST ATOM BOMBARDMENT COMBINED WITH TANDEM MASS-SPECTROMETRY FOR THE DETERMINATION OF NUCLEOSIDES [J].

CROW, FW ;

TOMER, KB ;

GROSS, ML ;

MCCLOSKEY, JA ;

BERGSTROM, DE .

ANALYTICAL BIOCHEMISTRY, 1984, 139 (01) :243-262

[4] FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY FOR STRUCTURAL ELUCIDATION OF GLYCOCONJUGATES [J].

EGGE, H ;

PETERKATALINIC, J .

MASS SPECTROMETRY REVIEWS, 1987, 6 (03) :331-393

[5] CHARACTERIZATION OF CYTOKININS FROM PLANT PATHOGENIC PSEUDOMONADES BY FAST-ATOM-BOMBARDMENT TANDEM MASS-SPECTROMETRY [J].

GRECO, F ;

SINDONA, G ;

UCCELLA, N ;

EVIDENTE, A .

ORGANIC MASS SPECTROMETRY, 1992, 27 (06) :750-755

[6]

Horgan R, 1987, PRINCIPLES PRACTICE, VII, P303

[7] DIRECT COUPLING OF MICRO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FAST ATOM BOMBARDMENT MASS-SPECTROMETRY [J].

ITO, Y ;

TAKEUCHI, T ;

ISHII, D .

JOURNAL OF CHROMATOGRAPHY, 1985, 346 (OCT) :161-166

[8] SEPARATION OF MODIFIED BASES AND RIBONUCLEOSIDES WITH CYTOKININ ACTIVITY USING FUSED-SILICA CAPILLARY GAS-CHROMATOGRAPHY [J].

KEMP, TR ;

ANDERSEN, RA .

JOURNAL OF CHROMATOGRAPHY, 1981, 209 (03) :467-471

[9] MASS-SPECTROMETRIC ANALYSIS OF CYTOKININS IN PLANT-TISSUE .7. QUANTIFICATION OF CYTOKININ BASES BY NEGATIVE-ION MASS-SPECTROMETRY [J].

LETHAM, DS ;

SINGH, S ;

WONG, OC .

JOURNAL OF PLANT GROWTH REGULATION, 1991, 10 (02) :107-113

[10]

Letham DS, 1978, PHYTOHORMONES RELATE, V1, P205

← 1 2 →