SILVER STAINING OF DNA IN POLYACRYLAMIDE GELS

被引：136

作者：

BASSAM, BJ

CAETANOANOLLES, G

机构：

[1] UNIV TENNESSEE, INST AGR, KNOXVILLE, TN 37901 USA

[2] UNIV TENNESSEE, CTR LEGUME RES, KNOXVILLE, TN 37901 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1993年 / 42卷 / 2-3期

关键词：

DNA; POLYACRYLAMIDE GELS; SILVER STAINING;

D O I：

10.1007/BF02788051

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleic acids can be detected at the picogram level using a quick and simple silver staining method (1). Using very thin polyester-backed polyacrylamide gels, a further simplified protocol was compared to other widely used silver staining procedures. The improved protocol described here was the most sensitive, the fastest to perform, and had relatively few steps and reagents. This method also produced the least number of staining artifacts and offered images of high contrast.

引用

页码：181 / 188

页数：8

共 12 条

[1]

ALLEN RC, 1989, BIOTECHNIQUES, V7, P736

[2] FAST AND SENSITIVE SILVER STAINING OF DNA IN POLYACRYLAMIDE GELS [J].