EFFECT OF DIABETES-MELLITUS ON RESPONSE OF THE BASILAR ARTERY TO ACTIVATION OF ATP-SENSITIVE POTASSIUM CHANNELS

Our goal was to determine whether responses of the basilar artery to activation of ATP-sensitive potassium channels are altered during diabetes mellitus. We measured changes in diameter of the basilar artery in vivo in non-diabetic and diabetic rats (streptozotocin; 50-60 mg/kg i.p.) in response to activation of ATP-sensitive potassium channels using aprikalim (RP 52891) and levcromakalim (BRL 38227). Aprikalim (1.0 mu M) dilated the basilar artery in non-diabetic rats by 27 +/- 6%, but by only 11 +/- 3% in diabetic rats (means +/- S.E.; P < 0.05). Levcromakalim (1.0 mu M) dilated the basilar artery in non-diabetic rats by 45 +/- 11%, but by only 20 +/- 5% in diabetic rats (P < 0.05). Nitroglycerin (1.0 mu M) dilated the basilar artery by 20 +/- 5% in non-diabetic rats and 17 +/- 2% in diabetic rats (P > 0.05). Thus, impaired dilatation of pial arterioles in diabetic rats in response to aprikalim and levcromakalim is not related to a non-specific effect of diabetes mellitus on vasodilatation. The findings of the present study suggest that ATP-sensitive potassium channels are functional in the rat basilar artery in vivo and are altered during diabetes mellitus.