SPLICING AND EDITING OF RPS10 TRANSCRIPTS IN POTATO MITOCHONDRIA

被引：41

作者：

ZANLUNGO, S ^{[1
]}

QUINONES, V ^{[1
]}

MOENNE, A ^{[1
]}

HOLUIGUE, L ^{[1
]}

JORDANA, X ^{[1
]}

机构：

[1] PONTIFICIA UNIV CATOLICA CHILE,FAC CIENCIAS BIOL,DEPT BIOL CELULAR & MOLEC,SANTIAGO,CHILE

来源：

CURRENT GENETICS | 1995年 / 27卷 / 06期

关键词：

GROUP-II INTRON EDITING; RPS10 TRANSCRIPT SPLICING; S10 RIBOSOMAL PROTEIN; SOLANUM TUBEROSUM MITOCHONDRIA;

D O I：

10.1007/BF00314449

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The structure and expression of the potato mitochondrial gene rps10, encoding ribosomal protein S10, has been characterized. The RPS10 polypeptide of 129 amino acids is encoded by two exons of 307 bp and 80 bp respectively, which are separated by a 774-bp class-II intron. Editing of the complete rps10 coding region was studied by sequence analysis of spliced cDNAs. Four C residues are edited into U, resulting in the creation of a putative translational initiation codon, a new stop codon which eliminated ten carboxy-terminal residues, and two additional amino-acid alterations. All these changes increase the similarity between the potato and liverwort polypeptides. One additional C-to-U RNA editing event, observed in the intron sequence of unspliced cDNAs, improves the stability of the secondary structure in stem I (i) of domain I and may thus be required for the splicing reaction. All spliced cDNAs, and most unspliced cDNAs, were completely edited, suggesting that editing is an early step of rps10 mRNA processing and precedes splicing. Earlier work on potato rps10 (Zanlungo et al. 1994) is now known to comprise only a partial analysis of the gene, since the short downstream exon was not identified.

引用

页码：565 / 571

页数：7

共 32 条

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