REMOVAL OF SODIUM DODECYL-SULFATE FROM PROTEINS ISOLATED BY SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS

A procedure is described for the removal of sodium dodecyl sulphate (SDS) from proteins isolated by SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE). The proteins separated by SDS‐PAGE were stained with Coomassie Blue and extracted with Tris‐HCI buffer containing SDS. The obtained extracts were subjected to gel permeation chromatography in an acidic aqueous acetonitrile solution. The procedure allows purification of the isolated proteins not only from SDS, but also from Coomassie Blue, buffer salts and other small molecular weight contaminants. Copyright © 1990 John Wiley & Sons, Ltd.