DIFFERENTIATION OF MONOCYTOID THP-1 CELLS WITH PHORBOL ESTER INDUCES EXPRESSION OF PROSTAGLANDIN ENDOPEROXIDE SYNTHASE-1 (COX-1)

被引:102
作者
SMITH, CJ
MORROW, JD
ROBERTS, LJ
MARNETT, LJ
机构
[1] VANDERBILT UNIV,MED CTR,SCH MED,DEPT BIOCHEM,CTR MOLEC TOXICOL,AB HANCOCK JR MEM LAB CANC RES,NASHVILLE,TN 37232
[2] VANDERBILT UNIV,MED CTR,SCH MED,DEPT MED,NASHVILLE,TN 37232
[3] VANDERBILT UNIV,MED CTR,SCH MED,DEPT PHARMACOL,NASHVILLE,TN 37232
关键词
D O I
10.1006/bbrc.1993.1483
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prostaglandin endoperoxide synthase (PGH synthase) is responsible for converting arachidonic acid to PGH2, the common precursor of prostaglandins. It has been shown previously that phorbol ester-induced differentiation of the THP-1 human monocytic leukemia cell line is accompanied by induction of PGH synthase enzyme and enhanced capacity to produce prostaglandins. However, the identity of the isoform of PGH synthase that is induced under these conditions has not been established. Northern and Western analyses revealed a dramatic increase in levels of PGH synthase-1 mRNA and protein levels within 24 hr after treatment of THP-l cells with phorbol ester. No significant increase in PGH synthase-2 mRNA or protein was observed. The increases in PGH synthase-l were accompanied by enhanced capacity of the cells to produce PGE2. These findings indicate that expression of PGH synthase-1 is greatly enhanced in THP-1 cells by treatment with an agent that induces differentiation. © 1993 Academic Press. All rights reserved.
引用
收藏
页码:787 / 793
页数:7
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