PURIFICATION AND PROPERTIES OF IMMUNOTOXINS CONTAINING ONE VS 2 DEGLYCOSYLATED RICIN-A CHAINS

被引：20

作者：

GHETIE, V

SWINDELL, E

UHR, JW

VITETTA, ES

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT MICROBIOL,DALLAS,TX 75235

[2] UNIV TEXAS,SW MED CTR,CTR CANC IMMUNOBIOL,DALLAS,TX 75235

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 166卷 / 01期

关键词：

RICIN A CHAIN; IMMUNOTOXIN; (ANTIBODY);

D O I：

10.1016/0022-1759(93)90335-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the preparation of immunotoxins (ITs) containing one and two molecules of deglycosylated ricin A chain (dgA) bound to each molecule of anti-human CD22 mouse IgG1 monoclonal antibody is described. The cross-linking of antibody and dgA molecules was accomplished with N-succinimidyl-oxycarbonyl-alpha-methyl-(2-pyridyldithio) toluene. The two ITs were purified by successive chromatographies on Blue-Sepharose, Sephacryl S-200, Blue-Sepharose with NaCl gradient and Sephacryl S-300. The IT with two dgAs was seven-fold more cytotoxic to CD22(+) Daudi cells than that containing one molecule of dgA.

引用

页码：117 / 122

页数：6

共 8 条

[1]

AMLOT PL, 1993, IN PRESS BLOOD

[2]

GHETIE MA, 1988, CANCER RES, V48, P2610

[3] THE GLP LARGE-SCALE PREPARATION OF IMMUNOTOXINS CONTAINING DEGLYCOSYLATED RICIN-A CHAIN AND A HINDERED DISULFIDE BOND [J].