CHOLINERGIC DIFFERENTIATION OF CULTURED SYMPATHETIC NEURONS INDUCED BY RETINOIC ACID - INDUCTION OF CHOLINE ACETYLTRANSFERASE-MESSENGER-RNA AND SUPPRESSION OF TYROSINE HYDROXYLASE-MESSENGER-RNA LEVELS

被引:50
作者
KOBAYASHI, M [1 ]
MATSUOKA, I [1 ]
KURIHARA, K [1 ]
机构
[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN
关键词
TRANSMITTER PHENOTYPE; SYMPATHETIC NEURON; CHOLINE ACETYLTRANSFERASE; TYROSINE HYDROXYLASE; RETINOIC ACID; POLYMERASE CHAIN REACTION;
D O I
10.1016/0014-5793(94)80204-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Here we show that retinoic acid (RA) has the ability to alter the transmitter phenotype of cultured sympathetic neurons from newborn rats superior cervical ganglia (SCG). In the presence of RA, the level of choline acetyltransferase (ChAT) mRNA was increased, while the level of tyrosine hydroxylase (TH) mRNA was reduced in the cultured SCG neurons. Selective PCR amplification of different upstream regions of the ChAT-mRNA indicates that RA promotes the transcription of ChAT gene from R and M exons. The RA-induced upregulation of ChAT-mRNA level was significantly diminished by the chronic treatment with phorbol eater, suggesting that PKC has an important role in the induction of ChAT-mRNA in this system.
引用
收藏
页码:259 / 264
页数:6
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