SOURCES OF HUMAN SCHWANN-CELLS AND THE INFLUENCE OF DONOR AGE

被引：16

作者：

BOYER, PJ

TUITE, GF

DAUSER, RC

MURASZKO, KM

TENNEKOON, GI

RUTKOWSKI, JL

机构：

[1] UNIV MICHIGAN,MED CTR,DEPT PEDIAT,ANN ARBOR,MI 48109

[2] UNIV MICHIGAN,MED CTR,DEPT NEUROL,ANN ARBOR,MI 48109

[3] UNIV MICHIGAN,MED CTR,DEPT PATHOL,ANN ARBOR,MI 48109

[4] UNIV MICHIGAN,MED CTR,DEPT NEUROSURG,ANN ARBOR,MI 48109

来源：

EXPERIMENTAL NEUROLOGY | 1994年 / 130卷 / 01期

关键词：

D O I：

10.1006/exnr.1994.1184

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We evaluated several tissues as possible sources for culturing human Schwann cells. The average cell yield (total cell number/mg of nerve fascicle) obtained from adult autopsy cases and transplant organ donors was similar (2 x 10(4) and 2.9 x 10(4), respectively), but significantly higher yields were obtained from dorsal roots of pediatric patients undergoing selective dorsal rhizotomy (6.1 x 10(4)). Fresh tissue was not essential since cells isolated from 0 to 20 h postmortem were equally viable. However, we found evidence that donor age affects the intrinsic growth rate of Schwann cells and perineurial fibroblasts in culture. (C) 1994 Academic Press, Inc.

引用

页码：53 / 55

页数：3

共 11 条

[1] HUMAN SCHWANN-CELLS IN TISSUE-CULTURE - HISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES [J].