COMPARISON OF BASIC FIBROBLAST GROWTH-FACTOR LEVELS IN CLONE-A HUMAN COLON-CANCER CELLS IN-VITRO WITH LEVELS IN XENOGRAFTED TUMORS

被引：6

作者：

MCCARTY, LP

KARR, SM

HARRIS, BZ

MICHELSON, SG

LEITH, JT

机构：

[1] BROWN UNIV,SCH MED,DEPT RADIAT MED,RADIAT RES LABS,PROVIDENCE,RI 02912

[2] SYNTEX CORP,DEPT BIOMATH,PALO ALTO,CA 94303

来源：

BRITISH JOURNAL OF CANCER | 1995年 / 72卷 / 01期

关键词：

BASIC FIBROBLAST GROWTH FACTOR; XENOGRAFTED TUMORS; HYPOXIA; HOST CELLS;

D O I：

10.1038/bjc.1995.269

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We measured levels of basic fibroblast growth factor (FGF-2) in human colon cancer cells (clone A) in vitro and in xenografted solid tumours using a commercial enzyme-linked immunoassay. In Vitro, levels in unfed plateau phase or exponentially growing cells were low, averaging respectively about 2 and 8 pg 10(-6) cells. However, when solid tumours (average volumes 787 mm(3)) were cut into halves and either enzymatically disaggregated to obtain a cellular fraction or extracted in toto, levels were much higher. In the cellular fraction, values averaged 110 pg 10(-6) cells, while in whole tumour extracts, average values were 24 pg mg(-1) tumour tissue. These results indicate that growth factor levels in solid neoplasms may differ markedly from those predicted from in vitro measurements. We hypothesise that the apparent increase in FGF-2 levels in vivo results primarily from the presence of a significant fraction of host cells (in particular, macrophages, which may contain high levels of FGF-2) within xenografted clone A neoplasms.

引用

页码：10 / 16

页数：7

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