IDENTIFICATION OF A NOVEL MAMMALIAN MEMBER OF THE NSF/CDC48P/PAS1P/TBP-1 FAMILY THROUGH HETEROLOGOUS EXPRESSION IN YEAST

被引：37

作者：

PERIER, F

COULTER, KL

LIANG, H

RADEKE, CM

GABER, RF

VANDENBERG, CA

机构：

[1] UNIV CALIF SANTA BARBARA,DEPT BIOL SCI,SANTA BARBARA,CA 93106

[2] UNIV CALIF SANTA BARBARA,NEUROSCI RES INST,SANTA BARBARA,CA 93106

[3] NORTHWESTERN UNIV,DEPT BIOCHEM MOLEC BIOL & CELL BIOL,EVANSTON,IL 60208

来源：

FEBS LETTERS | 1994年 / 351卷 / 02期

关键词：

ATPASE; MEMBRANE FUSION; YEAST; SUPPRESSOR; POTASSIUM TRANSPORT; TRK;

D O I：

10.1016/0014-5793(94)00879-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two suppressors of the growth deficiency of a potassium transport mutant of Saccharomyces cerevisiae were isolated from a mouse cDNA expression library. These suppressors, SKD1 and SKD2 (suppressor of K+ transport growth defect), were cDNAs encoding members of a family of ATPases involved in membrane fusion (N-ethylmaleimide-sensitive fusion protein, NSF), cell division cycle regulation (CDC48p), peroxisome assembly (Paslp), and transcriptional regulation (TBP-1). The SKD1 protein constitutes a novel member of this family with 49-58% amino acid sequence similarity with other family members, and contains a single ATP binding site. The SKD2 polypeptide is the mouse homolog of NSF.

引用

页码：286 / 290

页数：5

共 36 条

[1] DEPLETION OF INTRACELLULAR POTASSIUM DISRUPTS COATED PITS AND REVERSIBLY INHIBITS CELL POLARIZATION DURING FIBROBLAST SPREADING [J].