STUDIES ON NON-STARCHY POLYSACCHARIDES OF ENDOSPERM OF NAKED BARLEY .V. DEGRADATIONS OF F-1 BETA-GLUCAN WITH SOME CELLULASES

F-1 β-glucan, the main component of water soluble non-starchy polysaccharides from naked barley endosperm, has been subjected to degradation with cellulases from Trichoderma viride and Trametes sanguinea. The former cellulase converted F-1 β-glucan to d-glucose, celiobiose, 4-O-β-laminaribiosyl-d-glucose and 42-O-β-laminaribiosyl-cellobiose as main products. From the latter preparation four fractions of cellulase were separated. Their hydrolysing mechanisms against F-1 β-glucan differed from each other. Thus, it was suggested that the hydrolysing mechanism of cellulase was different when its origin or fraction in the same origin differed. Cellulase from Trichoderma viride was almost similar to those from Streptomyces spearated by Parrish et al. and from Aspergillus niger by Stone et al. about the hydrolysing mechanism on barley β-glucans, though a small difference was found. © 1968 Taylor and Francis Group LLC.