NECESSITY FOR 2 HUMAN-CHROMOSOMES FOR HUMAN CHORIONIC-GONADOTROPIN PRODUCTION IN HUMAN-MOUSE HYBRIDS

被引：25

作者：

BORDELONRISER, ME ^{[1
]}

SICILIANO, MJ ^{[1
]}

KOHLER, PO ^{[1
]}

机构：

[1] UNIV TEXAS,MD ANDERSON HOSP & TUMOR INST,DEPT BIOL,HOUSTON,TX 77030

来源：

SOMATIC CELL GENETICS | 1979年 / 5卷 / 05期

关键词：

D O I：

10.1007/BF01542697

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Through a series of human-mouse hybrids we have identified that two human chromosomes, 10 and 18, must be present for production of the pregnancy protein hormone human chorionic gonadotropin (hCG). Human choriocarcinoma cells producing hCG were hybridized to mouse cells. From 49 independent clones three hybrid clones continued to produce whole hCG. Chromosomal analysis was done on the 3 producer clones and 5 nonproducer clones. The additional 41 nonproducer clones were genetically characterized by isozymes. Only when chromosomes 10 and 18 were present in a clone would the whole hCG molecule be produced. Clones with only 10 or only 18 did not produce hormone. Nine subclones of a producer clone confirmed this observation. Three subclones retaining both 10 and 18 continued to produce hCG. This study demonstrated the need to use cellular chromosome data and population enzyme data to identify two chromosomes necessary for hCG production in heterogeneous human-mouse hybrids. © 1979 Plenum Publishing Corporation.

引用

页码：597 / 613

页数：17

共 38 条

[1] Rankin J., Darlington G., Somat. Cell Genet., 5, (1979)
[2] Bordelon M., Coon H., Kohler P., Exp. Cell Res., 103, (1976)
[3] Pierce J., Endocrinology, 89, (1971)
[4] Vaitukaitis J., Ross G., Gonadotropins, (1972)
[5] Ballon S., Berman M., Lagasse L., Watring W., Schlesinger R., Obstet. Gynecol. Survey, 32, (1977)
[6] Liddle G., Nichiolson W., Island D., Orth D., Abe K., Lowder S., Rec. Progr. Horm. Res., 25, (1969)
[7] Rosen S., Weintraub B., Vaitukaitis J., Sussman H., Hershamm J., Muggia F., Ann. Intern. Med., 82, (1975)
[8] Kohler P., Bridson W., Hammond J., Weintraub B., Kirschner M., Van Thiel D., Acta Endocrinol. Suppl., 153, (1971)
[9] Kohler P., Bridson W., J. Clin. Endocrinol., 32, (1971)
[10] Hertz R., Proc. Soc. Exp. Biol. Med., 102, (1969)

← 1 2 3 4 →