HYDROGEN AUTOTROPHY OF NOCARDIA-OPACA STRAINS IS ENCODED BY LINEAR MEGAPLASMIDS

被引：55

作者：

KALKUS, J ^{[1
]}

REH, M ^{[1
]}

SCHLEGEL, HG ^{[1
]}

机构：

[1] UNIV GOTTINGEN,INST MIKROBIOL,GRISEBACHSTR 8,W-3400 GOTTINGEN,GERMANY

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷

关键词：

D O I：

10.1099/00221287-136-6-1145

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Several linear megaplasmids were detected in the facultatively lithoautotrophic Gram-positive bacterium Nocardia opaca. The wild-type strain MR11 contains, in addition to the cccDNA plasmids pHG31-a and pHG31-b, the linear plasmids pHG201 (270 kb), pHG202 (400 kb) and pHG203 (420 kb). The wild-type strain MR22 contains, in addition to the cccDNA plasmid pHG33, the linear plasmids pHG204 (180 kb), pHG205 (280 kb) and pHG206 (510 kb). After preparation of DNA from cells embedded in agarose, the linear plasmids were demonstrated by pulsed-field electrophoresis. By means of DNA probes for genes of soluble hydrogenase and ribulose-bisphosphate carboxylase, the conjugative plasmids pHG201 and pHG205 were shown to be the carriers of the genetic information for these enzymes. A restriction map of pHG201 for the enzymes AsnI, SpeI, XbaI is presented.

引用

页码：1145 / 1151

页数：7

共 32 条

[1] STUDIES ON A GRAM-POSITIVE HYDROGEN BACTERIUM, NOCARDIA-OPACA STRAIN 1B .1. DESCRIPTION AND PHYSIOLOGICAL CHARACTERIZATION [J].

AGGAG, M ;

SCHLEGEL, HG .

ARCHIV FUR MIKROBIOLOGIE, 1973, 88 (04) :299-318

[2] PULSED FIELD GEL-ELECTROPHORESIS - A TECHNIQUE FOR FRACTIONATING LARGE DNA-MOLECULES [J].

ANAND, R .

TRENDS IN GENETICS, 1986, 2 (11) :278-283

[3] PLASMIDS REQUIRED FOR UTILIZATION OF MOLECULAR-HYDROGEN BY ALCALIGENES-EUTROPHUS [J].

ANDERSEN, K ;

TAIT, RC ;

KING, WR .

ARCHIVES OF MICROBIOLOGY, 1981, 129 (05) :384-390

[4] LINEAR PLASMIDS OF THE BACTERIUM BORRELIA-BURGDORFERI HAVE COVALENTLY CLOSED ENDS [J].

BARBOUR, AG ;

GARON, CF .

SCIENCE, 1987, 237 (4813) :409-411

[5] DETECTION OF SUBPICOGRAM QUANTITIES OF SPECIFIC DNA-SEQUENCES ON BLOT HYBRIDIZATION WITH BIOTINYLATED PROBES [J].

CHAN, VTW ;

FLEMING, KA ;

MCGEE, JOD .

NUCLEIC ACIDS RESEARCH, 1985, 13 (22) :8083-8091

[6] ISOLATION AND CHARACTERIZATION OF A LINEAR PLASMID FROM STREPTOMYCES-RIMOSUS [J].

CHARDONLORIAUX, I ;

CHARPENTIER, M ;

PERCHERON, F .

FEMS MICROBIOLOGY LETTERS, 1986, 35 (2-3) :151-155

[7] ENZYMES OF THE AUTOTROPHIC PATHWAY IN MATING PARTNERS AND TRANSCONJUGANTS OF NOCARDIA-OPACA-1B AND RHODOCOCCUS-ERYTHROPOLIS [J].

ECKER, C ;

REH, M ;

SCHLEGEL, HG .

ARCHIVES OF MICROBIOLOGY, 1986, 145 (03) :280-286

[8] NATURALLY-OCCURRING GENETIC TRANSFER OF HYDROGEN-OXIDIZING ABILITY BETWEEN STRAINS OF ALCALIGENES-EUTROPHUS [J].

FRIEDRICH, B ;

HOGREFE, C ;

SCHLEGEL, HG .

JOURNAL OF BACTERIOLOGY, 1981, 147 (01) :198-205

[9] PHYSICAL EVIDENCE FOR PLASMIDS IN AUTOTROPHIC, ESPECIALLY HYDROGEN-OXIDIZING BACTERIA [J].

GERSTENBERG, C ;

FRIEDRICH, B ;

SCHLEGEL, HG .

ARCHIVES OF MICROBIOLOGY, 1982, 133 (02) :90-96

[10] LINEAR PLASMID-LIKE DNA IN STREPTOMYCES SP PRODUCING LANKACIDIN GROUP ANTIBIOTICS [J].

HAYAKAWA, T ;

TANAKA, T ;

SAKAGUCHI, K ;

OTAKE, N ;

YONEHARA, H .

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1979, 25 (04) :255-260

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