PRODUCTION AND SECRETION OF C-19 STEROIDS BY RAT AND GUINEA-PIG ADRENALS

被引：41

作者：

BELANGER, B ^{[1
]}

COUTURE, J ^{[1
]}

CARON, S ^{[1
]}

BODOU, P ^{[1
]}

FIET, J ^{[1
]}

BELANGER, A ^{[1
]}

机构：

[1] CHU LAVAL,RES CTR,MOLEC ENDOCRINOL LAB,2705 LAURIER BLVD,QUEBEC CITY G1V 4G2,QUEBEC,CANADA

来源：

STEROIDS | 1990年 / 55卷 / 08期

关键词：

C-19; STEROIDS; ADRENALS; ANDROGENS; 11-BETA-HYDROXYANDROSTENEDIONE; ACTH;

D O I：

10.1016/0039-128X(90)90060-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The concentrations of C-19 steroids were measured in guinea pig and rat adrenals before and after castration as well as after stimulation with adrenocorticotropin hormone (ACTH). Characterization of adrenal C-19 steroid3 was also carried out by isolation with high-performance liquid chromatography and gas chromatography/mass spectrometry (GC/MS). From radioimmunoassay (RIA) data, androstenedione (4-DIONE) and 11-beta-hydroxyandrostenedione (11-beta-DIONE) were the major C-19 steroids found in guinea pig adrenals, and castration induced a decrease of 4-DIONE levels only while all other C-19 steroids remained unchanged. In rat adrenals, the major C-19 steroids were 4-DIONE and testosterone, and they were also markedly inhibited after castration. With the exception of 11-beta-DIONE, all other C-19 steroids in circulation were eliminated after castration in both animals species. After ACTH administration in the guinea pig, adrenal 4-DIONE and 11-beta-DIONE levels were markedly stimulated, while an increase of only 11-beta-DIONE was observed in plasma. In the rat, ACTH had a small stimulatory effect on adrenal 52-androstane-3-alpha, 17-beta-diol (3-alpha-DIOL) and plasma 11-beta-DIONE levels. Analysis of guinea pig adrenal steroids by GC/MS confirmed the presence of C-19 steroids in adrenals (namely, 4-DIONE and 11-beta-DIONE) while, in the rat, this could not be confirmed. Our data indicate that production of C-19 steroids occurs in guinea pig adrenals, and 11-beta-DIONE is the major C-19 steroid as well as the only C-19 steroid secreted into the circulation. In the rat, the production of C-19 steroids detected by RIA is not supported by GC/MS data.

引用

页码：360 / 365

页数：6

共 20 条

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