BOVINE LENS ALDOSE REDUCTASE - TIGHT-BINDING OF THE PYRIDINE COENZYME

被引:44
作者
DELCORSO, A
BARSACCHI, D
GIANNESSI, M
TOZZI, MG
CAMICI, M
HOUBEN, JL
ZANDOMENEGHI, M
MURA, U
机构
[1] UNIV PISA,DIPARTIMENTO FISIOL & BIOCHIM,VIA S MARIA 55,I-56100 PISA,ITALY
[2] UNIV PISA,DIPARTIMENTO CHIM & CHIM IND,I-56100 PISA,ITALY
[3] CNR,IST CHIM QUANTIST & ENERGET MOLEC,I-56100 PISA,ITALY
关键词
D O I
10.1016/0003-9861(90)90675-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Analysis by HPLC of the protein-free supernatant obtained after denaturation of aldose reductase shows that the native form of the enzyme (ARb) contains a tightly bound NADP+, which is absent in the oxidatively modified form (ARa). The absorption, fluorescence, and circular dichroism spectra of ARb and ARa are consistent with the presence of the cofactor only in the native form of aldose reductase. On the other hand, the modified enzyme, in appropriate thiol reducing conditions, can tightly bind NADP+. This indicates a potential reversibility of the modification of aldose reductase, at least in terms of retention of the cofactor. © 1990.
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页码:512 / 518
页数:7
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