IL-4 INHIBITS LPS-INDUCED, IL-1-BETA-INDUCED AND TNF-ALPHA-INDUCED EXPRESSION OF TISSUE FACTOR IN ENDOTHELIAL-CELLS AND MONOCYTES

Inflammatory mediators such as endotoxin, interleukin-1-beta(IL-1-beta) and tumor necrosis factor-alpha(TNF-alpha) dose-dependently increased the expression of tissue factor on the surface of cultured bovine aortic endothelial cells (ABAE), human umbilical vein endothelial cells (HUVEC) and human monocytes. In ABAE, endotoxin-, IL-1-beta- and TNF-alpha-induced tissue factor expression was suppressed by interleukin-4 (IL-4) which also neutralized the pyrogenic effect of endotoxin in HUVEC and monocytes. IL-4 did not alter TNF-alpha-induced procoagulant changes in HUVEC and monocytes but strongly protected the monocyte surface against IL-1-beta-induced procoagulant changes.