CAPSAZEPINE AS A SELECTIVE ANTAGONIST OF CAPSAICIN-INDUCED ACTIVATION OF C-FIBERS IN GUINEA-PIG BRONCHI

We investigated the action of capsazepine, an antagonist of the actions of capsaicin on sensory neurones, on the contractile responses evoked by capsaicin or by electrical field stimulation (EFS) in guinea-pig bronchi. Capsazepine (10(-5) M) selectively inhibited responses to capsaicin, producing a significant change in EC50 values but not the Hill coefficient (n(H)), suggesting that capsazepine acts as a competitive antagonist (apparent pK(B) = 5.12) whereas ruthenium red is a non-competitive antagonist. Capsazepine and ruthenium red were without effect on EFS-induced responses.