THE INCREASE IN GENE-EXPRESSION INDUCED BY INTRODUCTION OF RARE CODONS INTO THE C-TERMINUS OF THE TEMPLATE

被引：12

作者：

GURSKY, YG

BEABEALASHVILLI, RS

机构：

[1] Institute of Experimental Cardiology, Cardiology Research Center, Moscow, 121552

来源：

GENE | 1994年 / 148卷 / 01期

关键词：

RECOMBINANT DNA; TRANSLATION; TRANSFER RNA; CODON USAGE; RNA STABILITY; ESCHERICHIA-COLI;

D O I：

10.1016/0378-1119(94)90228-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Short oligodeoxynucleotides (oligos) possessing two tandem Arg codons followed by TGA stop codon were inserted near the 3' end of a modified cat gene. It was found that while being decoded in vivo, the AGGAGGTGA oligo increased the yield of gene product and, in addition, caused -1 frameshifting. The 3-10-fold increase of the yield of the polypeptide was accompanied by increased accumulation of corresponding mRNA, indicating a protection from messenger decay. Transformation of the cells by a plasmid overproducing tRNA(4)(Arg) gene compensates for all the anomalies.

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页码：15 / 21

页数：7

相关论文

共 29 条

[1] CODON PREFERENCES IN FREE-LIVING MICROORGANISMS [J].

ANDERSSON, SGE ;

KURLAND, CG .

MICROBIOLOGICAL REVIEWS, 1990, 54 (02) :198-210

[2] MECHANISMS OF MESSENGER-RNA DECAY IN BACTERIA - A PERSPECTIVE [J].

BELASCO, JG ;

HIGGINS, CF .

GENE, 1988, 72 (1-2) :15-23

[3] THE AGG CODON IS TRANSLATED SLOWLY IN ESCHERICHIA-COLI EVEN AT VERY LOW EXPRESSION LEVELS [J].

BONEKAMP, F ;

JENSEN, KF .

NUCLEIC ACIDS RESEARCH, 1988, 16 (07) :3013-3024

[4] HIGH-LEVEL EXPRESSION OF RECOMBINANT GENES IN ESCHERICHIA-COLI IS DEPENDENT ON THE AVAILABILITY OF THE DNAY GENE-PRODUCT [J].

BRINKMANN, U ;

MATTES, RE ;

BUCKEL, P .

GENE, 1989, 85 (01) :109-114

[5] CONSTRUCTION AND CHARACTERIZATION OF AMPLIFIABLE MULTICOPY DNA CLONING VEHICLES DERIVED FROM P15A CRYPTIC MINIPLASMID [J].

CHANG, ACY ;

COHEN, SN .

JOURNAL OF BACTERIOLOGY, 1978, 134 (03) :1141-1156

[6] SUPPRESSION OF THE NEGATIVE EFFECT OF MINOR ARGININE CODONS ON GENE-EXPRESSION - PREFERENTIAL USAGE OF MINOR CODONS WITHIN THE 1ST 25 CODONS OF THE ESCHERICHIA-COLI GENES [J].

CHEN, GFT ;

INOUYE, M .

NUCLEIC ACIDS RESEARCH, 1990, 18 (06) :1465-1473

[7] TRANSCRIPTION OF SINGLE-COPY HYBRID LACZ GENES BY T7 RNA-POLYMERASE IN ESCHERICHIA-COLI - MESSENGER-RNA SYNTHESIS AND DEGRADATION CAN BE UNCOUPLED FROM TRANSLATION [J].

CHEVRIERMILLER, M ;

JACQUES, N ;

RAIBAUD, O ;

DREYFUS, M .

NUCLEIC ACIDS RESEARCH, 1990, 18 (19) :5787-5792

[8] MACROMOLECULAR-COMPOSITION DURING STEADY-STATE GROWTH OF ESCHERICHIA-COLI B-R [J].

DENNIS, PP ;

BREMER, H .

JOURNAL OF BACTERIOLOGY, 1974, 119 (01) :270-281

[9] GROWTH-RATE-DEPENDENT ACCUMULATION OF 12 TRANSFER-RNA SPECIES IN ESCHERICHIA-COLI [J].

EMILSSON, V ;

NASLUND, AK ;

KURLAND, CG .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 230 (02) :483-491

[10] THE ESCHERICHIA-COLI DNAY GENE ENDCODES AN ARGININE TRANSFER-RNA [J].

GARCIA, GM ;

MAR, PK ;

MULLIN, DA ;

WALKER, JR ;

PRATHER, NE .

CELL, 1986, 45 (03) :453-459