ANALYSIS OF DNA FLANKING THE XINB LOCUS OF STREPTOMYCES-LIVIDANS REVEALS GENES ENCODING ACETYL XYLAN ESTERASE AND THE RNA COMPONENT OF RIBONUCLEASE-P

被引：40

作者：

SHARECK, F ^{[1
]}

BIELY, P ^{[1
]}

MOROSOLI, R ^{[1
]}

KLUEPFEL, D ^{[1
]}

机构：

[1] SLOVAK ACAD SCI,INST CHEM,BRATISLAVA,SLOVAKIA

来源：

GENE | 1995年 / 153卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

ACTINOMYCETES; HEMICELLULASES; GENE DUPLICATION; HOMOLOGY ANALYSIS; RNASE P; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(94)00763-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Nucleotide sequencing revealed the gene (axeA) encoding acetyl xylan esterase (AxeA) downstream from xlnB in the Streptomyces lividans DNA insert of plasmid pIAF42. AxeA consists of a catalytic- and a substrate-binding domain separated by a Gly-rich linker. The N terminus showed no significant homology with published esterases and acetyl xylan esterases, but some homology was found with the xylanases XylA and XylD and the NodB protein of Rhizobium species which is involved in the biosynthesis of root nodulation factors. The C terminus of AxeA is highly homologous to the C-termini of xylanases XlnB and TFXA, corresponding to the xylan-binding domain of these enzymes. Furthermore, the RNaseP RNA component was found immediately upstream from xlnB gene.

引用

页码：105 / 109

页数：5

共 22 条

[11] SOME PROPERTIES OF EXTRACELLULAR ACETYLXYLAN ESTERASE PRODUCED BY THE YEAST RHODOTORULA-MUCILAGINOSA [J].