STIMULATION OF DNA-SYNTHESIS IN CARCINOGEN-INDUCED DIPLOID HEPATOCYTES INVITRO

被引：8

作者：

KLOSE, U ^{[1
]}

THIERAU, D ^{[1
]}

VESER, U ^{[1
]}

SCHWARZ, LR ^{[1
]}

机构：

[1] GESELL FORSCH,INST TOXIKOL,INGOLSTADTER LANDSTR 1,W-8042 NEUHERBERG,GERMANY

来源：

CARCINOGENESIS | 1990年 / 11卷 / 09期

关键词：

D O I：

10.1093/carcin/11.9.1479

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Diploid hepatocytes induced by a combination of diethylnitrosamine and 2-acetylaminofluorene were isolated and separated from polyploid hepatocytes by centrifugal elutriation. The diploid and polyploid cell fractions were ∼90% pure and contained between 1 and 1.5×107 cells. When kept in monolayer cultures both cell populations responded to the mitogemc effect of EGF and insulin. However, the percentage of labelled nuclei was higher in predominantly diploid compared to predominantly tetraploid hepatocyte cultures at all epidermal growth factor (EGF) concentrations used in this study. At 10 ng EGF/ml and 10 mU insulin/ml the labelling index was twice as high in the diplold liver cells. Further work is required to show the relevance of the stronger response of the diploid cell fraction to mitogensin the process of carcinogenesis. © 1990 Oxford University Press.

引用

页码：1479 / 1483

页数：5

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