ALPHA-4 IS A MAJOR ACETYLCHOLINE BINDING SUBUNIT OF CHOLINERGIC LIGAND AFFINITY-PURIFIED NICOTINIC ACETYLCHOLINE-RECEPTOR FROM RAT BRAINS

被引：29

作者：

NAKAYAMA, H

NAKASHIMA, T

KUROGOCHI, Y

机构：

[1] Department of Pharmacology, Nara Medical University, Kashihara

来源：

NEUROSCIENCE LETTERS | 1991年 / 121卷 / 1-2期

关键词：

ACETYLCHOLINE RECEPTOR; NICOTINIC RECEPTOR; SUBUNIT STRUCTURE; RAT BRAIN;

D O I：

10.1016/0304-3940(91)90664-F

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Nicotinic acetylcholine receptor (nAChR) purified from rat brains by cholinergic ligand affinity chromatography was characterized. Monoclonal antibody 299, which binds an acetylcholine (ACh) binding subunit termed alpha-4, depleted more than 85% of [H-3]ACh binding activity of the purified preparation. A number of cholinergic agonists strongly inhibited [H-3]ACh binding to the purified nAChR, whereas potential antagonists were less effective than the agonists. These results show that most of the purified nAChR contains alpha-4 subunit and the pharmacological properties are preserved upon purification.

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页码：122 / 124

页数：3

相关论文

共 25 条

[1] ISOLATION OF A NICOTINE BINDING-SITE FROM RAT-BRAIN BY AFFINITY-CHROMATOGRAPHY [J].

ABOOD, LG ;

LATHAM, W ;

GRASSI, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (11) :3536-3539

[2] NICOTINE BINDING TO BRAIN-TISSUE FROM DRUG-NAIVE AND NICOTINE-TREATED RATS [J].

BENWELL, MEM ;

BALFOUR, DJK .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 1985, 37 (06) :405-409

[3] FUNCTIONAL EXPRESSION OF 2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS FROM CDNA CLONES IDENTIFIES A GENE FAMILY [J].

BOULTER, J ;

CONNOLLY, J ;

DENERIS, E ;

GOLDMAN, D ;

HEINEMANN, S ;

PATRICK, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7763-7767

[4] ISOLATION OF A CDNA CLONE CODING FOR A POSSIBLE NEURAL NICOTINIC ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT [J].

BOULTER, J ;

EVANS, K ;

GOLDMAN, D ;

MARTIN, G ;

TRECO, D ;

HEINEMANN, S ;

PATRICK, J .

NATURE, 1986, 319 (6052) :368-374

[5]

BOULTER J, 1990, J BIOL CHEM, V265, P4472

[6]

COSTA LG, 1983, J PHARMACOL EXP THER, V226, P392

[7] PRIMARY STRUCTURE AND EXPRESSION OF BETA-2 - A NOVEL SUBUNIT OF NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS [J].

DENERIS, ES ;

CONNOLLY, J ;

BOULTER, J ;

WADA, E ;

WADA, K ;

SWANSON, LW ;

PATRICK, J ;

HEINEMANN, S .

NEURON, 1988, 1 (01) :45-54

[8]

DENERIS ES, 1989, J BIOL CHEM, V264, P6268

[9] THE FUNCTIONAL DIVERSITY OF THE NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS IS INCREASED BY A NOVEL SUBUNIT - BETA-4 [J].

DUVOISIN, RM ;

DENERIS, ES ;

PATRICK, J ;

HEINEMANN, S .

NEURON, 1989, 3 (04) :487-496

[10] MEMBERS OF A NICOTINIC ACETYLCHOLINE-RECEPTOR GENE FAMILY ARE EXPRESSED IN DIFFERENT REGIONS OF THE MAMMALIAN CENTRAL-NERVOUS-SYSTEM [J].

GOLDMAN, D ;

DENERIS, E ;

LUYTEN, W ;

KOCHHAR, A ;

PATRICK, J ;

HEINEMANN, S .

CELL, 1987, 48 (06) :965-973