DEVELOPMENTAL-CHANGES IN PROLIFERATIVE ACTIVITY OF CELLS OF THE MURINE RATHKES POUCH

被引:54
作者
IKEDA, H
YOSHIMOTO, T
机构
[1] Division of Neurosurgery, Institute of Brain Diseases, Tohoku University School of Medicine, Sendai
关键词
LABELING INDEX; RATHKES POUCH; BROMODEOXYURIDINE; ACTH; RAT (WISTAR);
D O I
10.1007/BF00318398
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To clarify proliferative activity in the cells of the Rathke's pouch of the rat, we studied the labeling index using bromodeoxyuridine-immunohistochemistry. Rat fetuses were removed 1 h after transplacental injection of bromodeoxyuridine on day 11.5-21.5 of gestation, and were subsequently used to examine cellular proliferation. Although the labeling index within the Rathke's pouch was 30% on day 12.5, it decreased with development and by day 18.5 of gestation had a value of about 5%. The labeling index within Rathke's pouch was not homogenous throughout the entire pouch, but tended to be higher in regions where cells were more densely packed. This heterogeneous pattern of distribution of labeling index values continued until day 15. On that day, immunoreactive ACTH cells first appeared in the region where the labeling index was low. From day 17 of gestation, the uneven distribution of the labeling index became vague and, simultaneously, the distribution of ACTH cells became homogeneous throughout the pouch. It was concluded that proliferation of the epithelium of Rathke's pouch is heavily involved in the growth of the pouch until at least the appearance of ACTH cells.
引用
收藏
页码:41 / 47
页数:7
相关论文
共 10 条
[1]   TOPOGRAPHICAL APPEARANCE OF ADENOHYPOPHYSEAL CELLS WITH SPECIAL REFERENCE TO THE DEVELOPMENT OF THE PORTAL SYSTEM [J].
DAIKOKU, S ;
KAWANO, H ;
ABE, K ;
YOSHINAGA, K .
ARCHIVUM HISTOLOGICUM JAPONICUM, 1981, 44 (02) :103-116
[2]  
DAIKOKU S, 1986, PARS DISTALIS PITUIT, P21
[3]  
DOSKOCIL M, 1970, DEV CHICK HYPOPHYSIS
[4]  
DUPOUY JP, 1975, CELL TISSUE RES, V161, P373
[6]   USE OF AVIDIN-BIOTIN-PEROXIDASE COMPLEX (ABC) IN IMMUNOPEROXIDASE TECHNIQUES - A COMPARISON BETWEEN ABC AND UNLABELED ANTIBODY (PAP) PROCEDURES [J].
HSU, SM ;
RAINE, L ;
FANGER, H .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1981, 29 (04) :577-580
[7]   THE DEVELOPMENT AND MORPHOGENESIS OF THE HUMAN PITUITARY-GLAND [J].
IKEDA, H ;
SUZUKI, J ;
SASANO, N ;
NIIZUMA, H .
ANATOMY AND EMBRYOLOGY, 1988, 178 (04) :327-336
[8]  
SETALO G, 1976, ENDOCRINOL EXP, V10, P155
[9]  
SETALO G, 1972, ANAT REC, V172, P403
[10]   IMMUNOHISTOCHEMICAL STUDY ON THE CYTOGENESIS OF ADENOHYPOPHYSEAL CELLS IN FETAL RATS [J].
WATANABE, YG ;
DAIKOKU, S .
DEVELOPMENTAL BIOLOGY, 1979, 68 (02) :557-567