OLIGONUCLEOTIDE HYBRIDIZATIONS ON GLASS SUPPORTS - A NOVEL LINKER FOR OLIGONUCLEOTIDE SYNTHESIS AND HYBRIDIZATION PROPERTIES OF OLIGONUCLEOTIDES SYNTHESIZED INSITU

被引：282

作者：

MASKOS, U

SOUTHERN, EM

机构：

[1] Department of Biochemistry, University of Oxford, Oxford OX1 3QU, South Parks Road

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 07期

关键词：

D O I：

10.1093/nar/20.7.1679

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. These hybridisations were carried out with oligonucleotides synthesised on 'ballotini' solid sphere glass beads and microscope slides. The linker has a hexaethylene glycol spacer, bound to the glass via a glycidoxypropyl silane, terminating in a primary hydroxyl group that serves as starting point for automated or manual oligonucleotide synthesis.

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页码：1679 / 1684

页数：6

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