ORGANIZATION OF THE GENES ENCODING P-AMINOBENZOIC ACID SYNTHETASE FROM STREPTOMYCES-LIVIDANS 1326

被引：10

作者：

ARHIN, FF ^{[1
]}

VINING, LC ^{[1
]}

机构：

[1] DALHOUSIE UNIV,DEPT BIOL,HALIFAX B3H 4J1,NS,CANADA

来源：

GENE | 1993年 / 126卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

COMPLEMENTATION CLONING; P-AMINOBENZOATE AUXOTROPHS; NUCLEOTIDE SEQUENCES; HOMOLOGOUS INTEGRATION; HETEROLOGOUS EXPRESSION; PRIMARY METABOLISM;

D O I：

10.1016/0378-1119(93)90601-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Genes involved in the biosynthesis of p-aminobenzoic acid (PABA) in Streptomyces lividans 1326 were cloned in pBR322 by complementing a pabB mutant of Escherichia coli. A 2.7-kb BamHI-SstI fragment of the cloned DNA complemented pabA and pabB mutations in both E. coli and S. lividans; complementation in S. lividans was accompanied by integration of the recombinant plasmid into the host chromosome. The nucleotide (nt) sequence of the 2.7-kb fragment contained two open reading frames, the deduced amino acid sequences of which were similar to those of pabA and pabB products from other bacteria. The nt sequences indicated that pabA and pabB are closely linked in S. lividans and supported cloning evidence that the genes are expressed from a promoter with features resembling those of most E. coli promoters.

引用

页码：129 / 133

页数：5

共 28 条

[1] PLASMID TRANSFORMATION OF STREPTOMYCES-VENEZUELAE - MODIFIED PROCEDURES USED TO INTRODUCE THE GENE(S) FOR PARA-AMINOBENZOATE SYNTHASE [J].