NONEQUIVALENT NATURES OF THE COORDINATED IMIDAZOLE RINGS OF CYTOCHROME-C(3) FROM D-VULGARIS MIYAZAKI-F AS STUDIED BY H-3 NMR

All of the C2 proton signals of the coordinated histidine residues in the H-1 NMR spectrum of cytochrome c3 from D. vulgaris Miyazaki F were assigned by specific deuteration. They appeared at extremely high fields and scattered in a wide range from -4 to -22 ppm. This clearly shows that the chemical properties of the imidazole groups are quite different from one another. The extremely high-field shift of the C2 signal indicates that some of them must carry the imidazolate-like nature to some extent. This might be responsible for the extremely low redox potentials of the four hemes. On changing temperature, most of them showed Curie-type change. All of the C2 signals showed a small (pH)-H-2 dependence in the range of (pH)-H-2 4.8-10.0.