ACTIVATION OF CORD T-LYMPHOCYTES .3. ROLE OF LFA-1/ICAM-1 AND CD2/LFA-3 ADHESION MOLECULES IN CD3-INDUCED PROLIFERATIVE RESPONSE

被引:14
作者
GERLI, R
AGEA, E
MUSCAT, C
TOGNELLINI, R
FIORUCCI, G
SPINOZZI, F
CERNETTI, C
BERTOTTO, A
机构
[1] PERUGIA GEN HOSP,BLOOD BANK,PERUGIA,ITALY
[2] PERUGIA GEN HOSP,DIV NUCL MED,PERUGIA,ITALY
[3] UNIV PERUGIA,INST PEDIAT,I-06100 PERUGIA,ITALY
关键词
D O I
10.1006/cimm.1993.1089
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
As cord T cells, a model of antigen (Ag)-unprimed cell, display a functional defect when stimulated through the CD3 molecule, the role of lymphocyte function-associated antigen 1(LPA-1)/intercellular adhesion molecule 1 (ICAM-1) and CD2/lymphocyte function-associated antigen 3 (LFA-3) receptor-ligand pairs in cord CD3-triggered T-cell activation was analyzed using specific monoclonal antibodies (mAb) against each adhesion molecule. The addition of anti-CD11a, anti-CD18, or anti-CD2 to both adult and cord peripheral blood mononuclear cells (PBMC) cultures led to a decrease in CD3-induced proliferation. In contrast, CD3-stimulated cord, but not adult, PBMC proliferation was markedly enhanced when anti-CD54 or anti-CD58 were added. Despite the fact that ICAM-1 and LFA-3 molecules were virtually absent on cord resting T cells, mAb against these two molecules boosted both mitogenesis of and interleukin (IL)-2 production by purified cord T cells stimulated with plastic immobilized anti-CD3. Cord T-cell supernatant levels of interferon-γ (IFN-γ) were undetectable with CD3 stimulation, slightly raised with CD58/CD3 costimulation, but normal when T cells were preincubated with IL-2 for 24 hr before being costimulated with anti-CD3/CD58. Evidence that IL-2 and IFN-γ play a pivotal role in fully activating cord T cells came from the demonstration that IL-2 and IFN-γ are able to bypass the CD3-proliferative defect through differential up-regulation of the adhesion molecules. It would, therefore, seem that ICAM-1 and LFA-3 molecules are crucially implicated in the CD3-activation pathway of Ag-unprimed T cells.
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页码:32 / 47
页数:16
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