ANDROGEN METABOLISM ASSESSMENT BY ROUTINE GAS-CHROMATOGRAPHY MASS-SPECTROMETRY PROFILING OF PLASMA STEROIDS .1. UNCONJUGATED STEROIDS

被引：39

作者：

WUDY, SA ^{[1
]}

WACHTER, UA ^{[1
]}

HOMOKI, J ^{[1
]}

TELLER, WM ^{[1
]}

SHACKLETON, CHL ^{[1
]}

机构：

[1] CHILDRENS HOSP,OAKLAND RES INST,OAKLAND,CA

来源：

STEROIDS | 1992年 / 57卷 / 07期

关键词：

STEROIDS; ANDROGENS; PLASMA; DEUTERATED STEROIDS; GAS CHROMATOGRAPHY MASS SPECTROMETRY;

D O I：

10.1016/0039-128X(92)90050-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using gas chromatography/mass spectrometry we have developed a method for the simultaneous determination of six plasma steroids: testosterone, 4-androstenedione, 17-alpha-hydroxyprogesterone, 5-alpha-androstane-3-alpha,17-beta-diol, 5-alpha-dihydrotestosterone, and dehydroepiandrosterone. For each analyte, a deuterium-labeled internal standard was used for quantification. Due to the high isotopic purity of our standards, no complex corrections for isotope contributions were necessary. The procedure provides a sensitive and specific technique with good accuracy and precision.

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页码：319 / 324

页数：6

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