THE ESCHERICHIA-COLI VISA GENE ENCODES FERROCHELATASE, THE FINAL ENZYME OF THE HEME BIOSYNTHETIC-PATHWAY

被引:29
作者
FRUSTACI, JM
OBRIAN, MR
机构
[1] SUNY BUFFALO,DEPT BIOCHEM,140 FARBER HALL,BUFFALO,NY 14214
[2] SUNY BUFFALO,CTR ADV MOLEC BIOL & IMMUNOL,BUFFALO,NY 14214
关键词
D O I
10.1128/JB.175.7.2154-2156.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An Escherichia coli mutant with a disrupted visA gene was defective in ferrochelatase activity but expressed wild-type levels of protoporphyrinogen oxidase activity. The visA coding region was placed under the transcriptional control of T7 RNA polymerase in an E. coli expression system, and the product was expressed as a 38-kDa protein. The overexpressed protein was purified to near homogeneity and was found to contain ferrochelatase activity. The data show that the visA gene encodes ferrochelatase, and we propose that it be renamed hemH to reflect that conclusion.
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页码:2154 / 2156
页数:3
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