IMMOBILIZATION OF THIOBACILLUS-FERROOXIDANS USING VARIOUS POLYMERS AS MATRIX

被引:24
作者
WAKAO, N
ENDO, K
MINO, K
SAKURAI, Y
SHIOTA, H
机构
[1] Laboratory of Applied Microbiology, Department of Bioscience and Technology, Faculty of Agriculture, Iwate University
关键词
D O I
10.2323/jgam.40.349
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The cells of acidophilic iron-oxidizing bacteria (Thiobacillus ferrooxidans) were immobilized using five kinds of polymers (photo-crosslinkable resin, agar, calcium alginate, kappa-carrageenan, and gelrite) as gel matrix, and the characteristics of cell immobilization were revealed. Among these gels, gelrite showed promise for application in iron oxidation under strongly acidic conditions. In continuous cultures of iron-oxidizing bacteria immobilized in gelrite beads, a steady state of strong iron oxidation was maintained for a month. The thick layer of iron-oxide precipitates was formed around reddish-brown ''activated beads'' that harbored a large number of iron-oxidizing bacteria and were the sources of cells living in the liquid phase.
引用
收藏
页码:349 / 358
页数:10
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