COVALENT CHROMATOGRAPHY - PREPARATION OF FULLY ACTIVE PAPAIN FROM DRIED PAPAYA-LATEX

被引：238

作者：

BROCKLEHURST, K ^{[1
]}

CARLSSON, J ^{[1
]}

KIERSTAN, MP ^{[1
]}

CROOK, EM ^{[1
]}

机构：

[1] UNIV LONDON, ST BARTHOLOMEWS HOSP, MED COLL, DEPT BIOCHEM, LONDON EC1M 6BQ, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1973年 / 133卷 / 03期

关键词：

D O I：

10.1042/bj1330573

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：573 / 584

页数：12

共 29 条

[1] Arnon R., 1970, PAPAIN METHODS ENZYM, V19, P226, DOI DOI 10.1016/0076-6879(70)19017-3
[2] COVALENT AFFINITY COLUMN FOR PURIFICATION OF ACETYLCHOLINESTERASE
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[3] CHEMICAL COUPLING OF PEPTIDES AND PROTEINS TO POLYSACCHARIDES BY MEANS OF CYANOGEN HALIDES
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ERNBACK, S
[J]. NATURE, 1967, 214 (5095) : 1302 - &
[4] AXEN R, 1971, EUR J BIOCHEM, V18, P351
[5] DETERMINATION OF CONCENTRATION OF HYDROLYTIC ENZYME SOLUTIONS - ALPHA-CHYMOTRYPSIN TRYPSIN PAPAIN ELASTASE SUBTILISIN AND ACETYLCHOLINESTERASE
BENDER, ML
BEGUECAN.ML
BLAKELEY, RL
BRUBACHER, LJ
FEDER, J
GUNTER, CR
KEZDY, FJ
KILLHEFFER, JV
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MILLER, CG
ROESKE, RW
STOOPS, JK
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1966, 88 (24) : 5890 - +
[6] ISOLATION BY COVALENT AFFINITY CHROMATOGRAPHY OF PENICILLIN-BINDING COMPONENTS FROM MEMBRANES OF BACILLUS-SUBTILIS
BLUMBERG, PM
STROMING.JL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (12) : 3751 - 3755
[7] PURIFICATION OF PAPAIN BY AFFINITY CHROMATOGRAPHY
BLUMBERG, S
SCHECHTER, I
BERGER, A
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1970, 15 (01): : 97 - +
[8] REACTION OF PAPAIN WITH ELLMANS REAGENT [5,5'-DITHIOBIS-(2-NITROBENZOATE) DIANION]
BROCKLEHURST, K
LITTLE, G
KIERSTAN, M
[J]. BIOCHEMICAL JOURNAL, 1972, 128 (04) : 811 - +
[9] A NOVEL REACTIVITY OF PAPAIN AND A CONVENIENT ACTIVE SITE TITRATION IN PRESENCE OF OTHER THIOLS
BROCKLEHURST, K
LITTLE, G
[J]. FEBS LETTERS, 1970, 9 (02) : 113 - +
[10] REACTIONS OF PAPAIN AND OF LOW-MOLECULAR-WEIGHT THIOLS WITH SOME AROMATIC DISULFIDES - 2,2'-DIPYRIDYL DISULFIDE AS A CONVENIENT ACTIVE-SITE TITRANT FOR PAPAIN EVEN IN PRESENCE OF OTHER THIOLS
BROCKLEHURST, K
LITTLE, G
[J]. BIOCHEMICAL JOURNAL, 1973, 133 (01) : 67 - 80

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