CYTOGENETIC ANALYSIS OF UNCLEAVED OOCYTES AFTER INTRACYTOPLASMIC SPERM INJECTION

被引：17

作者：

BERGERE, M

SELVA, J

VOLANTE, M

DUMONT, M

HAZOUT, A

OLIVENNES, F

FRYDMAN, R

机构：

[1] IVF Laboratories and Gyneco-obstetric Department, Hôpital Antoine Béclère, Clamart

[2] Cytogenetic Unit, Hôpital Bicêtre, Kremlin Bicêtre

[3] Clinique Pierre Cherest, Neuilly

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 05期

关键词：

OOCYTE; SPERMATOZOA; CHROMOSOMES; MICROFERTILIZATION;

D O I：

10.1007/BF02213712

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: This work analyzes the causes of cleavage failure after intracytoplasmic sperm injection (ICSI) and the effect of the procedure on the chromosomes of the oocytes. Methods: Ninety-seven uncleaved oocytes from 39 patients with severe male infertility or repeated IVF failure were fixed; 79 were analyzable. We checked the decondensation stage of spermatozoa nucleus and the chromosomal abnormalities of the oocyres. Results: Among the fixed oocytes, the spermatozoa nucleus was present in 97% of the cases, and it was undecondensed in 89% of the cases, showing no evolution at all. A low rate (2.6%) of premature chromosome condensation (PCC) of the spermatozoa and a low rate (2.5%) of female diploidy were observed. Among the oocytes that could be karyotyped, we observed a high rare (45%) of chromosome breakage. Conclusion: ICSI fertilization failure was due mostly to the complete lack of evolution of the spermatozoa nucleus. Oocyte selection before ICSI seemed to lower the PCC rate. The high rate of oocyte chromosomal breakage rate has to be confirmed.

引用

页码：322 / 325

页数：4

共 13 条

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