PRODUCTION OF HUMAN PLASMA RETINOL-BINDING PROTEIN IN ESCHERICHIA-COLI

被引：9

作者：

WANG, TTY

LEWIS, KC

PHANG, JM

机构：

[1] Laboratory of Nutritional and Molecular Regulation, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, Building 434/2, Box B

来源：

GENE | 1993年 / 133卷 / 02期

关键词：

VITAMIN-A METABOLISM; RECOMBINANT DNA; PLASMA; PROTEIN PURIFICATION; NUTRIENT METABOLISM;

D O I：

10.1016/0378-1119(93)90654-L

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We designed a polymerase chain reaction (PCR) primer pair which allowed us to clone the cDNA coding for the human plasma retinol-binding protein (hRBP) into an Escherichia coli expression vector. Production of hRBP was confirmed by probing Western blots with antisera against plasma hRBP. Purification and characterization of the E. coli-produced plasma hRBP are also described. The availability of this expression system makes it possible to obtain large quantities of hRBP to facilitate our continuing studies of retinol and RBP metabolism.

引用

页码：291 / 294

页数：4

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