MOM22 IS A RECEPTOR FOR MITOCHONDRIAL TARGETING SEQUENCES AND COOPERATES WITH MOM19

被引:116
作者
MAYER, A
NARGANG, FE
NEUPERT, W
LILL, R
机构
[1] UNIV MUNICH, INST PHYSIOL CHEM PHYS BIOCHEM & ZELLBIOL, D-80366 MUNICH, GERMANY
[2] UNIV ALBERTA, DEPT BIOL SCI, EDMONTON, AB T6G 2E9, CANADA
关键词
MITOCHONDRIA; MOM22; MOM19; PRESEQUENCE RECEPTOR; PROTEIN IMPORT;
D O I
10.1002/j.1460-2075.1995.tb00094.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recognition of targeting signals is a crucial step in protein sorting within the cell, So far, only a few components capable of deciphering targeting signals have been identified, and insights into the chemical nature of the interaction between the signals and their receptors are scarce, Using highly purified mitochondrial outer membrane vesicles, we demonstrate that MOM22 and MOM19, components of the protein import complex of the outer membrane, bind preproteins at the mitochondrial surface in a reversible fashion, Interaction specifically and directly occurs with the N-terminal presequence and is abolished after inactivation of either MOM22 or MOM19, Binding is salt sensitive, suggesting that recognition involves electrostatic forces between the positive charges of the presequence and the acidic cytosolic domain of MOM22, MOM19 and MOM22 can be cross-linked with high efficiency, We propose that the two proteins form a complex which functions as the presequence receptor at the mitochondrial surface and facilitates the movement of preproteins into the translocation pore.
引用
收藏
页码:4204 / 4211
页数:8
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