EFFECT OF FREE FATTY-ACID ANION ON THE INVITRO BINDING OF 1-ANILINO-8-NAPHTHALENE SULFONATE TO CYTOPLASMIC PROTEINS FROM RAT-LIVER

被引：3

作者：

CHAKRABARTI, S ^{[1
]}

CHARESTBOULE, L ^{[1
]}

BRODEUR, J ^{[1
]}

机构：

[1] UNIV MONTREAL,FAC MED,DEPT PHARMACOL,MONTREAL 101,QUEBEC,CANADA

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1979年 / 24卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0009-2797(79)90104-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of oleate ion, a free fatty acid anion, on the binding characteristics of 1-anilno-8-naphthalene sulfonate (ANS) with the cytoplasmic proteins (Y and Z) from rat liver has been examined using fluorescence spectroscopy. ANS binds strongly with both ligandin (Y) and Z protein at a single binding site with dissociation constants of 0.6 and 1.4 μM respectively. Increasing concentrations of oleate ion decreased the ANS binding with either protein by competing with the ANS binding site. Relative binding constant of oleate ion for the hepatic ligandin or Z protein was about 2 μM as determined from the competitive inhibition of ANS binding. These results suggest that variations in the hepatic cytoplasmic free fatty acid concentration may be important in regulating the capacity of Y and Z proteins to transport other organic anions. © 1979.

引用

页码：83 / 93

页数：11

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