A DNA-BINDING PROTEIN IS REQUIRED FOR TERMINATION OF TRANSCRIPTION BY RNA POLYMERASE-I IN XENOPUS-LAEVIS

被引:43
作者
MCSTAY, B
REEDER, RH
机构
[1] Basic Sciences Division, Hutchinson Cancer Res. Center, Seatle, WA 98104
关键词
D O I
10.1128/MCB.10.6.2793
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We describe a partially fractionated in vitro transcription system from Xenopus laevis for the assay of transcription termination by RNA polymerase I. Termination in vitro was found to require a specific terminator sequence in the DNA and a DNA-binding protein fraction that produces a footprint over the terminator sequence.
引用
收藏
页码:2793 / 2800
页数:8
相关论文
共 19 条