PASSIVE RELEASE OF MONOCLONAL-ANTIBODIES FROM HYBRIDOMA CELLS

被引：7

作者：

MOHAN, SB

LYDDIATT, A

机构：

[1] School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT

来源：

CYTOTECHNOLOGY | 1991年 / 5卷 / 03期

关键词：

CONTINUOUS CULTURE; MONOCLONAL ANTIBODIES; PASSIVE MCAB RELEASE;

D O I：

10.1007/BF00556290

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Evidence is presented for the passive release of monoclonal antibodies (MCAB) from hybridoma cells grown in either batch or continuous-flow culture. This release is promoted at room temperature. Passively released MCAB is indistinguishable from that released by actively growing cells, as judged by SDS-polyacrylamide gel electrophoresis. The significance of these observations in relation to the continuous culture of hybridoma cells is discussed. Maximum MCAB content of TB/C3 hybridoma cells is about 55pg per cell, any additional MCAB produced is secreted.

引用

页码：201 / 209

页数：9

共 5 条

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