A NEW BIOLOGICAL-ACTIVITY OF THE COMPLEMENT FACTOR-H - IDENTIFICATION OF THE PRECURSOR OF THE MAJOR MACROPHAGE-CHEMOTACTIC FACTOR IN DELAYED-HYPERSENSITIVITY REACTION SITES OF GUINEA-PIGS

The guinea pig complement factor H(FH) and the plasma precursor(PMCFS-1) of the major monocyte-chemotactic factor(MCFS-1) found in the skin site of delayed hypersensitivity reaction(DHR) induced in the guinea pigs were compared in the antigenicity and the function. Both anti-FH-IgG and anti-MCFS-1-IgG formed a single precipitation line against FH, PMCFS-1, MCFS-1 and guinea pig plasma, and these lines fused one another without any spur formation. The inhibition activity of FH for C3bBb was absorbed by anti-MCFS-1-F(ab′)2 in a dose-dependent manner. PMCFS-1 inhibited C3bBb activity dose-dependently as FH. These results show that FH is identical to PMCFS-1 and imply that FH, converted to MCFS-1 plays as a monocyte-chemotactic factor in the site of DHR. © 1992.