PACKAGING CELLS FOR AVIAN-LEUKOSIS VIRUS-BASED VECTORS WITH VARIOUS HOST RANGES

被引：31

作者：

COSSET, FL ^{[1
]}

RONFORT, C ^{[1
]}

MOLINA, RM ^{[1
]}

FLAMANT, F ^{[1
]}

DRYNDA, A ^{[1
]}

BENCHAIBI, M ^{[1
]}

VALSESIA, S ^{[1
]}

NIGON, VM ^{[1
]}

VERDIER, G ^{[1
]}

机构：

[1] ECOLE NORMALE SUPER LYON, INRA, BIOL MOLEC & CELLULAIRE LAB, CNRS, UMR 49, F-69364 LYON 7, FRANCE

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 09期

关键词：

D O I：

10.1128/JVI.66.9.5671-5676.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using our previously described Haydee semipackaging cell line (F. L. Cosset, C. Legras, Y. Chebloune, P. Savatier, P. Thoraval, J. L. Thomas, J. Samarut, V. M. Nigon, and G. Verdier, J. Virol. 64:1070-1078, 1990) which produces avian leukosis virus gag and pol proteins, we have constructed packaging cells with subgroups B, C, and E envelope specificities. This allows us to produce helper-free avian leukosis virus particles carrying the lacZ reporter gene and the A, B, C, or E subgroup specificities. Titers of the recombinant lacZ virus are shown to be dependent upon the type of the env subgroup and the target avian cell.

引用

收藏

页码：5671 / 5676

页数：6

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