3' TRUNCATED TRANSFER-RNA (ARG) IS ESSENTIAL FOR IN-VITRO SPECIFIC CLEAVAGE OF PARTIALLY SYNTHESIZED MOUSE 18S RIBOSOMAL-RNA

被引：24

作者：

NASHIMOTO, M ^{[1
]}

机构：

[1] HOKKAIDO UNIV,SCH MED,DEPT BIOCHEM,SAPPORO,HOKKAIDO 060,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 20期

关键词：

D O I：

10.1093/nar/21.20.4696

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro synthesized 5' portions of mouse 18S rRNA are cleaved efficiently at a specific site in partially purified extracts of mouse FM3A cells and several mouse tissues. This activity is composed of both protein and RNA, and can be reconstituted with the protein component in micrococcal nuclease-treated extracts and the RNA component in phenol-treated ones. The RNA component of about 65 nucleotides with the complementing activity was purified from total RNA in the partially purified FM3A cell extracts by polyacrylamide gel electrophoreses. Chemical sequencing of this RNA elucidated that it is tRNA(Arg) lacking nine nucleotides from its 3' terminus. Ribonuclease H treatment directed by deoxyoligonucleotides complementary to tRNA(Arg) completely abolishes the cleavage activity, supporting the above conclusion.

引用

页码：4696 / 4702

页数：7

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