ASSOCIATION OF FLAVIN ADENINE-DINUCLEOTIDE WITH THE ARABIDOPSIS BLUE-LIGHT RECEPTOR CRY1

被引：364

作者：

LIN, CT

ROBERTSON, DE

AHMAD, M

RAIBEKAS, AA

JORNS, MS

DUTTON, PL

CASHMORE, AR

机构：

[1] UNIV PENN, INST PLANT SCI, DEPT BIOL, PHILADELPHIA, PA 19104 USA

[2] UNIV PENN, SCH MED, JOHNSON FDN, DEPT BIOCHEM & BIOPHYS, PHILADELPHIA, PA 19104 USA

[3] HAHNEMANN UNIV, SCH MED, DEPT BIOL CHEM, PHILADELPHIA, PA 19102 USA

来源：

SCIENCE | 1995年 / 269卷 / 5226期

关键词：

D O I：

10.1126/science.7638620

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 include an unexpected stability of the neutral radical flavosemiquinone (FADH .). The absorption properties of this flavosemiquinone provide a likely explanation for the additional sensitivity exhibited by CRY1-mediated responses in the green region of the visible spectrum, Despite the sequence homology to microbial DNA photolyases, CRY1 was found to have no detectable photolyase activity.

引用

页码：968 / 970

页数：3

共 26 条

[1] HY4 GENE OF A-THALIANA ENCODES A PROTEIN WITH CHARACTERISTICS OF A BLUE-LIGHT PHOTORECEPTOR
AHMAD, M
CASHMORE, AR
[J]. NATURE, 1993, 366 (6451) : 162 - 166
[2] Briggs W. R., 1976, Light and plant development. [Smith, H.]., P7
[3] DARWIN C, 1881, POWER MOVEMENT PLANT, P462
[4] Dutton P L, 1978, Methods Enzymol, V54, P411
[5] EKER APM, 1990, J BIOL CHEM, V265, P8009
[6] Galland Paul, 1991, P65
[7] RIBOFLAVIN, LIGHT, AND THE GROWTH OF PLANTS
GALSTON, AW
[J]. SCIENCE, 1950, 111 (2893) : 619 - 624
[8] BLUE-LIGHT PHOTORECEPTION
GRESSEL, J
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1979, 30 (06) : 749 - 754
[9] HANGARTER R, COMMUNICATION
[10] IDENTIFICATION OF A NEUTRAL FLAVIN RADICAL AND CHARACTERIZATION OF A 2ND CHROMOPHORE IN ESCHERICHIA-COLI DNA PHOTOLYASE
JORNS, MS
SANCAR, GB
SANCAR, A
[J]. BIOCHEMISTRY, 1984, 23 (12) : 2673 - 2679

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