PRODUCTION OF LONG-TERM STEROID-PRODUCING GRANULOSA-CELL CULTURES BY CELL HYBRIDIZATION

被引:19
作者
ZELEZNIK, AJ
HILLIER, SG
KNAZEK, RA
ROSS, GT
COON, HG
机构
[1] UNIV PITTSBURGH, MAGEE WOMENS HOSP, DEPT OBSTET & GYNECOL, PITTSBURGH, PA 15213 USA
[2] NIAMDD, DIABET BRANCH, BETHESDA, MD 20014 USA
[3] NICHHD, REPROD RES BRANCH, BETHESDA, MD 20014 USA
[4] NCI, PATHOPHYSIOL LAB, BETHESDA, MD 20014 USA
[5] NIH, CTR CLIN, BETHESDA, MD 20014 USA
[6] NCI, CELL BIOL LAB, BETHESDA, MD 20014 USA
关键词
D O I
10.1210/endo-105-1-156
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Primary cultures of rat ovarian granulosa cells have been used extensively to study hormonal regulation of cellular function. To date, no long term cultures of ovarian cells which retain their differentiated functions have been developed. Hypoxanthine guanine phosphoribosyl transferase-deficient simian virus 40-transformed rat ovarian granulosa cells were fused with freshly prepared rat granulosa cells using inactivated Sendai virus. Putative hybrid cell strains obtained after selection in medium containing hypoxanthine, aminopterin, and thymidine were analyzed for progesterone synthesis. Neither the original simian virus 40-transformed granulosa cell nor its hypoxanthine guanine phosphoribosyl transferase-deficient derivative produced progesterone, but three of the hybrid strains produced progesterone at basal levels and in response to dibutyryl cAMP. One of these strains produced progesterone in a dose-responsive fashion when exposed to prostaglandin E2, cholera toxin, dibutyryl cAMP, and 2-chloroadenosine. Cell strains obtained by hybridization were remarkably similar to primary cultures of granulosa cells with respect to both the magnitude and temporal aspects of progesterone production in response to dibutyryl cAMP. © 1979 by The Endocrine Society.
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收藏
页码:156 / 162
页数:7
相关论文
共 28 条
[1]   COLUMN CHROMATOGRAPHY OF STEROIDS ON SEPHADEX LH-20 [J].
CARR, BR ;
MIKHAIL, G ;
FLICKINGER, GL .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1971, 33 (02) :358-+
[2]  
Channing C P, 1970, Recent Prog Horm Res, V26, P589
[3]   CHARACTERISTICS OF GONADOTROPIN RECEPTORS OF PORCINE GRANULOSA CELLS DURING FOLLICLE MATURATION [J].
CHANNING, CP ;
KAMMERMAN, S .
ENDOCRINOLOGY, 1973, 92 (02) :531-540
[4]  
CHANNING CP, 1975, P SOC EXP BIOL MED, V149, P238
[5]   A QUANTITATIVE COMPARISON OF FORMATION OF SPONTANEOUS AND VIRUS-PRODUCED VIABLE HYBRIDS [J].
COON, HG ;
WEISS, MC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 62 (03) :852-&
[6]   HORMONE REQUIREMENTS FOR EARLY MAINTENANCE OF RAT GRANULOSA-CELL CULTURES [J].
CRISP, TM .
ENDOCRINOLOGY, 1977, 101 (04) :1286-1297
[7]   HEMOGLOBIN SYNTHESIS IN SOMATIC-CELL HYBRIDS - COEXPRESSION OF MOUSE WITH HUMAN OR CHINESE-HAMSTER GLOBIN GENES IN INTERSPECIFIC SOMATIC-CELL HYBRIDS OF MOUSE ERYTHROLEUKEMIA CELLS [J].
DEISSEROTH, A ;
BARKER, J ;
ANDERSON, WF ;
NIENHUIS, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (07) :2682-2686
[8]   PREGNENOLONE RADIOIMMUNOASSAY UTILIZING A NEW FRACTIONATION TECHNIQUE FOR SHEEP ANTISERUM [J].
DIPIETRO, DL ;
STROTT, CA ;
BROWN, RD .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1972, 35 (05) :729-+
[9]   RECOMBINATION AND SEGREGATION IN SOMATIC CELL HYBRIDS [J].
ENGEL, E ;
MCGEE, BJ ;
HARRIS, H .
NATURE, 1969, 223 (5202) :152-&
[10]   STIMULATORY ACTION OF GONADOTROPINS AND PROSTAGLANDINS ON ADENOSINE-3',5'-MONOPHOSPHATE PRODUCTION BY ISOLATED RAT GRANULOSA-CELLS [J].
GOFF, AK ;
ARMSTRONG, DT .
ENDOCRINOLOGY, 1977, 101 (05) :1461-1467