EFFECTS OF THIONINS ON BETA-GLUCURONIDASE INVITRO AND IN PLANT-PROTOPLASTS

被引：22

作者：

DIAZ, I

CARMONA, MJ

GARCIAOLMEDO, F

机构：

[1] UNIV POLITECN MADRID,ESCUELA TECN SUPER INGN AGRON,BIOQUIM & BIOL MOLEC LAB,E-28040 MADRID,SPAIN

[2] CSIC,CTR NACL BIOTECNOL,MADRID 6,SPAIN

来源：

FEBS LETTERS | 1992年 / 296卷 / 03期

关键词：

BETA-GLUCURONIDASE; THIONIN; TOBACCO PROTOPLAST; TRANSIENT EXPRESSION;

D O I：

10.1016/0014-5793(92)80304-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thionins cause the irreversible inactivation of beta-glucuronidase (GUS) in vitro in a dose- and time-dependent manner. The enzyme is also sensitive to externally added thionins when expressed in the cytoplasmic compartment of tobacco protoplasts transformed with the Gus gene under the 35S promoter of the cauliflower mosaic virus. In protoplasts transformed with the Gus gene fused to a signal peptide, where GUS is translocated into the lumen of the endoplasmic reticulum, the activity is significantly increased both by externally-added and by transiently-expressed thionin, suggesting that it interferes with GUS secretion.

引用

页码：279 / 282

页数：4

共 19 条

[1] LEAF-SPECIFIC THIONINS OF BARLEY - A NOVEL CLASS OF CELL-WALL PROTEINS TOXIC TO PLANT-PATHOGENIC FUNGI AND POSSIBLY INVOLVED IN THE DEFENSE-MECHANISM OF PLANTS [J].