SYNTHESIS AND CHARACTERIZATION OF A FRAGMENT OF AN ICE NUCLEATION PROTEIN

被引:7
作者
ALA, P
CHONG, P
ANANTHANARAYANAN, VS
CHAN, N
YANG, DSC
机构
[1] MCMASTER UNIV,DEPT BIOCHEM,HAMILTON L8N 3Z5,ONTARIO,CANADA
[2] CONNAUGHT RES INST,N YORK M2R 3T4,ON,CANADA
来源
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1993年 / 71卷 / 5-6期
关键词
ICE NUCLEATION PROTEIN; SYNTHETIC PEPTIDES; CIRCULAR DICHROISM;
D O I
10.1139/o93-036
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Synthetic peptides were used as models for studying the conformation of ice nucleation proteins. We chemically synthesized four peptides (16-, 24-, 32-, and 48-mer) that consisted of two to six repeats of the consensus repeating octapeptide unit of ice nucleation proteins and evaluated their conformation by circular dichroism spectroscopy. These model peptides exist predominantly as random coils in aqueous solution, but adopt alpha-helical structures in the presence of trifluoroethanol. The stability of their secondary structures was investigated by monitoring the pH and time of their circular dichroism spectra. Our results indicated that the alpha-helical content of the 48-mer exhibited a significant pH dependence, while that of the 24- and 32-mer peptides did not. The 32-mer was the only peptide that transformed from the alpha-helical to beta-sheet structure upon storage. We suggest that the overall conformation of the ice nucleation protein could be a beta-sheet.
引用
收藏
页码:236 / 240
页数:5
相关论文
共 19 条
[1]  
CHAKRABARTTY A, 1989, J BIOL CHEM, V264, P11307
[2]   DETERMINATION OF HELIX AND BETA-FORM OF PROTEINS IN AQUEOUS-SOLUTION BY CIRCULAR-DICHROISM [J].
CHEN, YH ;
YANG, JT ;
CHAU, KH .
BIOCHEMISTRY, 1974, 13 (16) :3350-3359
[3]   ICE NUCLEATION ACTIVITY OF PSEUDOMONAS-FLUORESCENS - MUTAGENESIS, COMPLEMENTATION ANALYSIS AND IDENTIFICATION OF A GENE-PRODUCT [J].
COROTTO, LV ;
WOLBER, PK ;
WARREN, GJ .
EMBO JOURNAL, 1986, 5 (02) :231-236
[4]   THE ROLE OF HEMOLYMPH-PROTEINS IN THE COLD TOLERANCE OF INSECTS [J].
DUMAN, J ;
HORWATH, K .
ANNUAL REVIEW OF PHYSIOLOGY, 1983, 45 :261-270
[5]   DELETION MUTAGENESIS OF THE ICE NUCLEATION GENE FROM PSEUDOMONAS-SYRINGAE S203 [J].
GREEN, RL ;
COROTTO, LV ;
WARREN, GJ .
MOLECULAR & GENERAL GENETICS, 1988, 215 (01) :165-172
[6]   COMPUTED CIRCULAR DICHROISM SPECTRA FOR EVALUATION OF PROTEIN CONFORMATION [J].
GREENFIE.N ;
FASMAN, GD .
BIOCHEMISTRY, 1969, 8 (10) :4108-&
[7]   DEVELOPMENT, DISTRIBUTION, AND CHARACTERISTICS OF INTRINSIC, NONBACTERIAL ICE NUCLEI IN PRUNUS WOOD [J].
GROSS, DC ;
PROEBSTING, EL ;
MACCRINDLEZIMMERMAN, H .
PLANT PHYSIOLOGY, 1988, 88 (03) :915-922
[8]   PROTEIN-INTERACTION WITH ICE [J].
HEW, CL ;
YANG, DSC .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 203 (1-2) :33-42
[9]   SOLID PHASE PEPTIDE SYNTHESIS .1. SYNTHESIS OF A TETRAPEPTIDE [J].
MERRIFIELD, RB .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (14) :2149-&
[10]   PREDICTION OF THE CONFORMATION OF ICE-NUCLEATION PROTEIN BY CONFORMATIONAL ENERGY CALCULATION [J].
MIZUNO, H .
PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1989, 5 (01) :47-65