P53 IMMUNOLOCALIZATION IN CYTOLOGY SPECIMENS - A STUDY IN HUMAN ESOPHAGEAL-CARCINOMA

被引：5

作者：

GOUKON, Y

SASANO, H

NISHIHIRA, T

MORI, S

NAGURA, H

机构：

[1] Departments of Pathology and Surgery, Tohoku University School of Medicine, Sendai

来源：

DIAGNOSTIC CYTOPATHOLOGY | 1994年 / 11卷 / 02期

关键词：

TUMOR SUPRESSOR GENE; GENE PRODUCT; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; SMEAR;

D O I：

10.1002/dc.2840110203

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Immunohistochemical analysis of p53 has become an established method of detecting mutated p53. Immunolocalization of p53 has been extensively studied in tissue sections of many human neoplasms, but not in cytology specimens. Therefore, we performed immunolocalization of p53 in both cytology and tissue preparations obtained from the same specimens in 19 cases of human esophageal squamous-cell carcinoma. Three different antibodies, PAb1801, DO-7, and CM-1, were employed Positive p53 nuclear immunoreactivity was observed in carcinoma cells, but not in noncarcinomatous cells, in both tissue and cytology preparations. The incidence of p53 positivity was 53% (10/19) in cytology and 42% (8/19) in tissue, and the coincident rate of both results was 89% (17/19). We conclude that immunohistochemistry of p53 in cytology is a useful method for detecting p53 mutations, and may contribute to the diagnosis of malignancy, because it is a simple, easy, and rapid technique. (C) 1994 Wiley-Liss, Inc.

引用

页码：113 / 118

页数：6

共 25 条

[1] Porter PL, Gown AM, Kramp SG, Coltrera MD, Widespread p53 overexpression in human malignant tumors, Am J Pathol, 140, pp. 145-153, (1992)
[2] Iggo R, Gatter K, Bartek J, Et al., Increased expression of mutant forms of p53 oncogene in primary lung cancer, Lancet, 335, pp. 675-679, (1990)
[3] Van Den Berg FM, Tigges AJ, Schipper MEI, Et al., Expression of the nuclear oncogene p53 in colon tumors, J Pathol, 157, pp. 193-199, (1989)
[4] Bartek J, Iggo R, Gannon J, Lane DP, Genetic and immunohistochemical analysis of mutant p53 in human breast cancer cell lines, Oncogene, 5, pp. 893-899, (1990)
[5] Tamura G, Kihara T, Nomura K, Et al., Detection of frequent p53 gene mutations in primary gastric cancer by cell sorting and polymerase chain reaction single‐strand conformation polymorphism analysis, Cancer Res, 51, pp. 3056-3058, (1991)
[6] Hollstein MC, Metcalf RA, Welsh JA, Et al., Frequent mutation of p53 gene in human esophageal cancer, Proceedings of the National Academy of Sciences, 87, pp. 9958-9961, (1990)
[7] Rogel A, Popliker M, Webb CG, p53 cellular tumor antigen
[8] analysis of mRNA levels in normal adult tissues, embryos and tumors, Mol Cell Biol, 5, pp. 2851-2855, (1985)
[9] Finlay CA, Hinds PW, Tan T-H., Activating mutations for transformation by p53 produce a gene product that forms an hsc70‐p53 complex with an altered half life, Mol Cell Biol, 8, pp. 531-539, (1988)
[10] Nochomovitz L, Sidaway M, Silverberg SG, Et al., Intraoperative consultation, a guide to smears, imprints and frozen sections, (1989)

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